What is likely to happen to transcription of the zippy gene

Cells have several mechanisms to prevent the production and accumulation of truncated protein fragments. To illustrate why these fragments can be deleterious, consider a transcriptional activator protein called Groovy that binds to an enhancer element upstream of the Zippy gene. The Groovy has 2 domains, a DNA-binding domain at its N-terminus that binds to DNA at the Zippy gene and a domain that binds a histone-modifying enzyme at its C-terminus. When the second domain binds a histone-modifying enzyme, the histone modifying enzyme alters the chromatin structure at the Zippy gene leading to increased transcription of Zippy.

A. If a cell makes substantial amounts of an N-terminal fragment of Groovy, containing the DNA-binding domain alone, what is likely to happen to transcription of the Zippy gene (increase, decrease or stay the same)?

B. If, in aIDition to the N-terminal fragment of Groovy, a cell also makes an equal amount of the full-length protein, what is likely to happen to transcription of the Zippy gene (increase, decrease or stay the same)?

C. Consider two cell lines or strains that each have a fully functional allele of the Groovy gene on one copy of chromosome 3. On the second copy of chromosome 3, strain 1 lacks a second Groovy allele whereas strain 2 contains the Groovy allele that codes for the N-terminal fragment described in part A. If strain 1 and strain 2 have different amounts of Zippy mRNA, which do you expect to have more Zippy mRNA? Explain.

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