Appropriate dilution scheme

Assume you were given a tube having 1 ml of frozen Staphylococcus epidermidis culture known to contain 5.3 x 103 bacteria/ml. You were told to thaw it by heating at 80°C for 20 min, and then prepare a 10-2 dilution.

a) Show an appropriate dilution scheme.

b) How many colonies do you expect to find on the plate?

 

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